Isithombe: I-Minimalist Laboratory Flask ene-Yeast Culture

Isithombe sibonisa ukulungiselelwa kwelabhorethri ecwengisiswe kakhulu, esezingeni elincane, eklanyelwe ukugqamisa kokubili izimfanelo zesayensi nezobuhle zomsebenzi wesiko lemvubelo. Maphakathi nendawo yokuqamba kukhona iflaski le-Erlenmeyer elihlanzekile elakhiwe ngengilazi ye-borosilicate ekhanyayo. Umzimba wayo oyindilinga uncipha kahle uye ngasentanyeni eyicylindrical, obonisa ukusebenza kwawo njengomkhumbi waselabhorethri wakudala. I-flask igcwele uketshezi oluphuzi ngokuphaphathekile, ukucaca kwalo kugcizelelwa ukukhanya okulinganayo, okuthambile okugeza yonke indawo. Ngaphakathi koketshezi, amafomu amancane amisiwe avusa ukuba khona kwamaseli emvubelo—ayindilinga, abonakala kancane kancane, futhi ahlakazeke ngamaphethini acashile, ngeqoqo elilodwa elikhulisiwe eliboniswe ngemininingwane emfishane ukuze kugcizelelwe isihloko sebhayoloji. Ukubuka kwangaphakathi kunikezwa ngobukhali obumangalisayo, okumema ukubhekisiswa ngokucophelela kwamaseli nokuhlelwa kwawo kuyilapho kugcinwa ubuqiniso besayensi obuhlanzekile.

Indawo okubekwe phezu kwayo iflaski iyindiza ebushelelezi, emhlophe ebonisa ngokucashile isisekelo sengilazi. Lokhu kubonakaliswa akuphazamisi kodwa kunalokho kuhambisana nokucaca okuphelele nokuzola kokusetha, okuqinisa itimu yokunemba okungenanyumba ehlobene nomsebenzi waselabhorethri. Ingemuva liyi-gradient empunga ethulisiwe, isuka kancane iye kumathoni amnyama kancane ngaphandle kokwethula iziphazamiso. Lokhu okwasemuva okuphakathi nendawo kuvumela iflaski nokuqukethwe kwayo ukuma njengendawo yokugxila engenakuphikiswa yokuqamba.

Ukukhanyisa kudlala indima ebalulekile esithombeni. Ukukhanya kungokwemvelo futhi kusabalele, njengokungathi kuhlungwe ngefasitela elinesithwathwa, akuvezi izithunzi ezinokhahlo kodwa kuthuthukisa i-contour yengilazi kanye nemibala ethambile yoketshezi. Ukukhanya kugqamisa ukugoba okubushelelezi kweflask, i-meniscus encane yoketshezi, namaseli eyeast antantayo abonakala elenga ngokulingana okuthulile. Ukusebenzisana kokukhanya nokukhanyela kunikeza indawo yesehlakalo ukuzola, i-aura yokucabangela, kuyilapho ngesikhathi esisodwa kudlulisa umuzwa wokunemba kanye nokuqina kobuchwepheshe.

Ubuhle buyinto encane ngamabomu—azikho izinto ezingaphandle, amalebula, noma imidwebo engena ekwakhiweni. Ngokugwema imfuhlumfuhlu ebonakalayo, isithombe sithwebula ingqikithi yokunemba kwesayensi: ukugxila kuhlala ngokuphelele kusiko lemvubelo, umkhumbi owuqukethe, kanye nendawo elawulwayo ehlala kuyo. Ubulula benkundla yokubukwayo buvusa kokubili ukuhlanzeka nokuhleleka, izimfanelo ezibalulekile emsebenzini waselabhorethri, kuyilapho kugcizelela ubucayi bezobuchwepheshe bokuhlaziywa kohlobo lwe-yeast.

Sekukonke, isithombe sikhuluma okungaphezu nje kwesifinyezo seflask yaselabhorethri; ihlanganisa izimiso zendlela yesayensi—ukucaca, ukunemba, ukuphindaphinda, nokugxila. Ilinganisa ubuciko nesayensi, yethula isiko lemvubelo hhayi nje kuphela njengesifundo se-microbiological kodwa futhi njengento ebukekayo ethule. Kungumfanekiso wokuhlola okulawulwayo, lapho ngisho nezilwanyana ezincane kakhulu ziphathwa ngokunaka nangenhlonipho lapho kufunwa ulwazi. Lokhu kwakheka okucatshangelwe kahle kuwuphawu lwamaphrofayili wobuchwepheshe athuthukiswe ocwaningweni lwe-yeast strain, okumema ababukeli ukuthi bathokozele ukusebenzisana komsebenzi, ifomu, nokutholwa.

Isithombe sihlobene: Ukuvubela Ubhiya Nge-White Labs WLP550 Belgian Ale Yeast