Isithombe: Amaseli Eyeast Asebenzayo kuPetri Dish

Lesi sithombe sinikeza amazwibela amangalisayo emhlabeni we-microscopic wokuvutshelwa, lapho ibhayoloji nekhemistri kuhlangana khona ngendlela ethambile, enyakazayo. Phakathi nendawo yokubunjwa kunesitsha se-petri, ifomu layo eliyindilinga eligcwele izakhi ezisagolide ezinsundu ezikhanya ngokufudumele ngaphansi kokukhanya okuthambile, okuqondisayo kwelabhorethri. Kulengiswe ngaphakathi kwalesi sikhungo kukhona inqwaba yamakholomu amise okweqanda, cishe amaseli emvubelo, ahlelwe ngendlela eguquguqukayo, evunguzayo evusa kokubili ubuhle bemvelo kanye nobuqili besayensi. Ukucushwa okujikelezayo kwamakholoni akusikiseli ukukhula okusebenzayo kuphela kodwa futhi nokusabela okungenzeka kuma-gradients emvelo-ukutholakala kwezakhi, izinga lokushisa, noma ukuhlushwa komoya-mpilo-okudala ukubonakaliswa okubonakalayo kokuziphatha kwe-microbial ngesikhathi sangempela.

Ama-yeast cells ngokwawo abonakala ephila futhi eqinile, ukwakheka kwawo kuchazwe kahle futhi ukusatshalaliswa kwawo phakathi nendawo kokubili kuminyene futhi kunenjongo. Amanye amakholomu ahlangana aqine, akhe amagquma akhiwe ngendlela ephakama kancane ngaphezu kwendawo, kuyilapho amanye asakazeka kakhulu, emaphethelweni awo anezimpaphe futhi angavamile. Lokhu kwehluka kwe-morphology kusikisela ubunkimbinkimbi benqubo yokuvutshelwa, lapho ukusho kofuzo, izinga lokugayeka kokudla, kanye nokuxhumana phakathi kwamangqamuzana ahlukene konke kunendima ekulolongeni ukwakheka kwekoloni. Umbala osagolide wendawo emaphakathi, othuthukiswa ukukhanya okufudumele, wengeza umuzwa wokunotha namandla endaweni yesehlakalo, uphakamisa i-substrate esekwe kwi-malt evamile yokuvutshelwa kukabhiya noma indawo efanayo enomsoco eklanyelwe ukusekela ukwanda kwemvubelo.

Isitsha se-petri sihlezi endaweni ehlanzekile, enensimbi ebonisa ukukhanya okuzungezile ngokukhazimula okucashile, okuqinisa imvelo eyinyumba, elawulwayo yesilungiselelo selabhorethri. Lesi sangemuva esishelelezi siphambene nobunkimbinkimbi bezinto eziphilayo zamakoloni amancane, okubonisa ukuphambana kobunjiniyela bomuntu kanye nokuziphendukela kwemvelo kwezinto eziphilayo. Ukujula kwenkundla okungajulile kuhlukanisa isitsha se-petri endaweni esizungezile, kudonsela iso lesibukeli emininingwaneni eyinkimbinkimbi yokwakheka kweyeast kuyilapho kuvumela ingemuva ukuthi lifiphale libe ukufiphala okuthambile. Amacebo ezinto zengilazi zaselabhorethri kanye nezinto zokusebenza—mhlawumbe amaflask, ama-pipette, noma amashidi edatha—ziyabonakala kodwa azibambeki, zengeza umongo ngaphandle kokuphazamiseka.

Isimo sesimo sesithombe singesokubuza okugxilile kanye nenhlonipho ethule. Kuthatha isikhathi lapho ama-ejenti angabonakali okuvutshelwa enziwa abonakale, umsebenzi wawo uqandiswe ngesikhathi sokutadisha nokwazisa. Iphethini ejikelezayo yamakholoni iphakamisa ukunyakaza nokuguqulwa, isikhumbuzo sokuthi ukuvutshelwa akuyona inqubo emile kodwa ukuxhumana okuguquguqukayo kokukhula, umzimba, nokuzivumelanisa nezimo. Ivusa ubuciko bokwenza isiphuzo, lapho ukukhethwa nokutshalwa kohlobo lweyeast kungaba nethonya elimangalisayo ukunambitheka, iphunga, nokuthungwa, nalapho ikoloni ngalinye limelela umnikelo omncane womkhiqizo wokugcina.

Ekugcineni, lesi sithombe siwumkhosi wokugubha impilo ye-microbial kanye nokuphishekela kwesayensi ukuyiqonda. Ngokwakheka kwayo, ukukhanya, kanye nemininingwane, imema umbukeli ukuthi abhekisise, acabangele inkimbinkimbi engemuva kwebhamuza ngalinye le-carbonation noma inothi lokunambitheka esiphuzweni esibilile. Kuwumfanekiso wokuvutshelwa hhayi nje njengenqubo, kodwa njengesimiso esiphilayo—esakheka kokubili izinto ezincane ezingaphakathi kwesitsha se-petri kanye nengqondo yomuntu ezifunda ngazo.

Isithombe sihlobene: Ukuvubela Ubhiya Nge-CellarScience German Yeast