Isithombe: Imvubelo Culture Analysis in the Lab

Lesi sithombe sithwebula isikhashana sophenyo lwesayensi olugxilile ngaphakathi kwelabhorethri ehlelwe kahle, lapho imingcele phakathi kwe-microbiology nesayensi yokuphisa ifiphala ibe yinto eyodwa, enenjongo. Enkabeni yokwakheka kukhona isazi sezinto eziphilayo ezincane, esigqoke ijazi lelebhu elihlanzekile, izibuko zokuphepha, namagilavu—into ngayinye yezingubo eziqinisa imvelo eyinyumba, elawulwayo yemvelo. Usosayensi uhlola ngokucophelela isitsha se-petri, esibanjwe kahle ngezandla ezifakwe amagilavu, kuyilapho sibekwe eceleni kwesibonakhulu esiyinhlanganisela. Ukuma nokugxilisa ingqondo kuphakamisa ukusebenzelana okujulile nesampula, okungenzeka isiko lamaseli emvubelo asebenzayo acutshungulwa nge-microscopic. Isitsha se-petri ngokwaso, nakuba sisincane, sinokubaluleka okukhulu kakhulu: ngaphakathi kwendilinga yaso kukhona iqoqo elichumayo lama-microorganisms, iseli ngalinye linikela ku-symphony eyinkimbinkimbi ye-biochemical of fermentation.

Ukukhanyisa ekamelweni kuhlanzekile futhi kwasakazwa ngokulinganayo, kukhipha ukukhanya okungathathi hlangothi endaweni yonke futhi kuqeda izithunzi ezinokhahlo. Lokhu kucaca kuthuthukisa ukubonakala kwemininingwane emihle—kusukela ekuthunjweni kwe-agar esitsheni se-petri kuya ekukhanyeni okucashile kumalensi e-microscope. Ukukhanya nakho kunomthelela esimweni somtholampilo, kugcizelela ukunemba nokuhlanzeka okudingekayo emsebenzini we-microbiological. Ibhentshi lelebhu elingaphambili alinayo imfuhlumfuhlu, nokho ligcwele amathuluzi abalulekile: amapayipi, amashubhu okuhlola, neziqukathi eziyinyumba, ngasinye siwumzila wokulinganisa, ukudlulisa, noma okokuqukatha. Lawa mathuluzi akhuluma ngokuqina kwenqubo yomsebenzi owenziwayo, lapho isinyathelo ngasinye sibhalwe phansi, konke okuguquguqukayo kulawulwa.

Phakathi nendawo, okokusebenza okwengeziwe njenge-incubator namabhodlela e-reagent aphakamisa ukuthi ukuhlaziya kuyingxenye yohlaka lokuhlola olubanzi. I-incubator, okungenzeka isetshenziselwa ukutshala amasiko emvubelo ngaphansi kwezimo ezithile zokushisa, ikhombisa ukubaluleka kokulawulwa kwemvelo ekukhuleni kwamagciwane. Ukuba khona kweziqukathi ezilebulwe nama-rack ahleliwe kuqinisa umqondo wokuthi lokhu akukhona ukubonwa kanye nje, kodwa kuyingxenye yocwaningo oluhlelekile—mhlawumbe umthetho olandelwayo wokulawula ikhwalithi wezinhlobo ze-yeast ezisetshenziswa ekuvubeleni kukabhiya. Imvubelo ehlolwayo ingase ihlolwe ukusebenza kahle, ukuhlanzeka, noma umsebenzi we-metabolic, konke okubalulekile ekuqinisekiseni imiphumela engaguquki nefiselekayo ekwenzeni utshwala.

Ingemuva lengeza ukujula nomongo esigcawini. Amashalofu ahlanganiswe namajenali esayensi, izincwadi eziyizithenjwa, namathuluzi okuhlaziya asikisela indawo ecwile olwazini nocwaningo oluqhubekayo. Lezi zinto azihlobisi; zimelela ukuhlakanipha okuqoqiwe kwesayensi yokuvutshelwa, okutholakalayo ukuze kuboniswane futhi kuqhathaniswe. Ukuba khona kwezibophezelo namafayela anelebuli kusho ukuthi idatha iyarekhodwa futhi ifakwe kungobo yomlando, okunikela endikimbeni ekhulayo yobufakazi obazisa amaqoqo azayo, ukukhethwa kohlobo, nokuthuthukiswa kokucubungula.

Sekukonke, isithombe sidlulisa umoya wokukhuthala okuthule kanye nelukuluku lobuhlakani. Kuwumfanekiso kasosayensi osebenzayo—hhayi eyedwa, kodwa njengengxenye yesimiso semvelo esikhulu samathuluzi, ulwazi, nenjongo. Ukugxila kumvubelo, i-microorganism evame ukunganakwa ngokuvuna izithako ezikhangayo zokuphisa, iphakamisa indima yayo njenge-ejenti eyinhloko yenguquko. Ngokubhekisisa nokuhlaziya, isazi sezinto ezincane eziphilayo siqinisekisa ukuthi ingqamuzana ngalinye lenza umsebenzi walo ngokunemba, okunezela ekunambitheka, iphunga, kanye nesimo somkhiqizo wokugcina. Lesi sigameko siwumkhosi wokugubha umsebenzi ongabonakali ngemuva kwawo wonke amaphayinti, futhi isikhumbuzo sokuthi ubhiya omkhulu awuqali nje endaweni yokuphuzela utshwala, kodwa elebhu—lapho isayensi ihlangana khona nobuciko ukuze iphishekele ubuhle.

Isithombe sihlobene: Ukuvubela Ubhiya nge-Lallemand LalBrew Abbaye Yeast