Isithombe: Brewer sika Imvubelo Lag Phase Culture

Isithombe sibonisa ukubuka okuseduze, okuseduze kwesiko lemvubelo yabaphisi botshwala esigabeni se-lag, esithwetshulwe ngaphakathi kwesitsha se-Petri esingashoni, esiyindilinga esibekwe endaweni yaselabhorethri ebhalwe ngokucashile. Konke ukubunjwa kufakwe ukukhanya okuthambile, okufudumele okubonakala kuvela engeli eliphansi kuya kwesokunxele, kudala izithunzi ezinde, ezithambile ezigcizelela isimo sezinhlangothi ezintathu nokuthungwa kwendawo ye-yeast colony. Ukujula kwenkambu okungajulile kunikeza ingemuva lingagxili, okuvumela iso ukuthi lidonswe ngokuphelele eqoqweni elimaphakathi leyeast, elibonakala licishe libazwe esakhiweni salo.

Isitsha sePetri ngokwaso senziwe ngengilazi ecacile noma ipulasitiki ekhanyayo, enemiphetho ebushelelezi, eyindilinga ebamba futhi ehlanekezela ukukhanya okufudumele kube amaphuzu avelele asagolide. Isitsha siqukethe ungqimba oluncane lwe-agar medium ephaphathekile, ubuso balo bushelelezi, bumanzi, futhi bukhanya kancane. Emaphethelweni esitsha, i-agar ishintsha ngobuqili isuka ku-beige eshintshashintshayo iye ethoni ejulile kancane eduze komphetho ngenxa yokuhlangana kokukhanya nesithunzi. Le gradient ecashile inikela kumqondo ophelele wokujula nokuba ngokoqobo endaweni yesehlakalo.

Enkabeni yesidlo kukhona isiko lemvubelo, esesigabeni sokuqala sokukhula okusebenzayo. Ikoloni eliyinhloko lakha indunduma eminyene, efana nedome, eyakhiwe amakholoni amancane ahlanganiswe ngokuqinile. Umbala wayo unokhilimu omhlophe ophuzi kanye nokufiphaza kophondo lwendlovu oluphaphathekile kanye ne-beige efudumele lapho ukukhanya kushaya khona ngokuqondile. Ingaphezulu linobuhlalu, obucishe bube nobuhlalu, obunama-globular amancane amancane abonisa amaphuzu avelele, aphakamisa amaqoqo amaseli emvubelo ngamanye aqala ukukhukhumala nokuhlukana. Amaphethelo angaphandle endunduma ashintsha ukusuka kuma-granules ahlanganiswe ngokuqinile ukuya kokuxega, amaseli angawodwana ahlakazeke kakhulu nama-microcoloni, abonisa ukusabalala kwangaphandle kokuqala ukusuka endaweni yokugonywa.

Ezungeza indunduma emaphakathi, ehlakazekile kuyo yonke i-agar, kukhona amakoloni amancane noma amaqoqo. Lawa abonakala njengamachashazi ahlukene, anosayizi wekhanda likaphini, futhi anokhilimu ngombala kodwa anezindawo ezibushelelezi kanye nokukhululeka okuphansi kancane kunekholoni eyinhloko. Ukuhlukaniswa kwawo kuphakamisa ukukhula kwesathelayithi yokuqala noma amaseli aseqalile ukuhluma ngemva kokujova kokuqala. Zinyamalala kancane zibe ingemuva elingagxilile, zenze i-organic gradient ukusuka kokuminyene kuye kokuncane okuqinisa umuzwa wokwanda kancane kancane kwe-microbial.

Ukukhanyisa ohlangothini kuyisihluthulelo somoya wesithombe. Ishushuluzela esitsheni nge-engeli ephansi, igqamisa ama-microtexture ngenkathi igwema ukukhanya okunamandla. Lokhu kukhanyisa kukhiqiza ukukhanya kwe-amber efudumele onqenqemeni lwesitsha nasendaweni ecwebezelayo ye-agar, kuyilapho kuveza izithunzi ezinhle ngaphansi kwekoloni ngalinye elincane. Lezi zithunzi zisiza ukucacisa izakhiwo ngazinye futhi zinikeze indawo iqiniso elithintekayo. Ukukhanya sekukonke kuthambile futhi kuthulisiwe kunokuba kube umtholampilo noma oyinyumba, okugcwalisa isithombe ngethoni yokucabanga efanele ukubhekwa kwesayensi nezinqubo zebhayoloji zesigaba sangaphambi kwesikhathi.

Ngemuva, indawo yaselabhorethri iyanyamalala ibe ukufiphala okuthambile, okuvelile, umbala wayo omaphakathi onsundu ngokumpunga oqinisekisa ukuthi ayiqhudelani ngokunakwa nesitsha. Lesi sangemuva esilufifi sinikeza kokubili ukugqama okubonakalayo nokujula, okwenza i-yeast culture egxile kakhulu igqame njengesihloko esicacile.

Sekukonke, isithombe sithwebula umzuzwana wokulindela okuthulile kwebhayoloji—iphuzu lapho amangqamuzana imvubelo evuka khona ngokomzimba kodwa angakabonakali aphindaphindeka ngesivinini esigcwele. Iveza ngokubonakalayo umqondo wesigaba se-lag ngokucacile okumangalisayo, ihlanganisa ubuqiniso besayensi nobuhle obufudumele, obucishe bube bobuciko.

Isithombe sihlobene: Ukubilisa Ubhiya nge-Lallemand LalBrew Diamond Lager Yeast